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Analysis of SEC-SAXS data via EFA deconvolution and Scatter
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Analysis of SEC-SAXS data via EFA deconvolution and Scatter

Analysis of SEC-SAXS data via EFA deconvolution and Scatter

DOI:
10.3791/61578-v

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10:59 min

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January 28, 2021

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, , ,
1European Synchrotron Radiation Facility Structural Biology Group,Structural Biology Group, 2Institut de Biologie Structurale, University Grenoble Alpes, CEA, CNRS, 3Diamond Light Source, 4Laboratoire de Physiologie Cellulaire and Végétale,Université Grenoble Alpes/CNRS/CEA/INRA/BIG

Chapters

  • 00:05Introduction
  • 00:49Primary Data Analysis
  • 02:20Data Deconvolution
  • 04:56Small Angle X-Ray Scattering (SAXS) Property Assessment
  • 08:22Results: Representative EFA Deconvolution and Scatter
  • 10:05Conclusion

Summary

Automatic Translation

Automatic Translation

SEC-BioSAXS measurements of biological macromolecules are a standard approach for determining solution structure of macromolecules and their complexes. Here, we analyze SEC-BioSAXS data from two types of commonly encountered SEC traces—chromatograms with fully resolved and partially resolved peaks. We demonstrate the analysis and deconvolution using scatter and BioXTAS RAW.

Tags

SEC-SAXSEFA DeconvolutionScatterVaccinia Virus DNA PolymeraseBackground SubtractionSize Exclusion ChromatographyMonodispersityMacromoleculeMacromolecular ComplexDeconvolutionData Analysis

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