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Ultra-High-Speed Western Blot ved hjælp af immunoreaktionsfremmende teknologi
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Biochemistry
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JoVE Journal Biochemistry
Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology

Ultra-High-Speed Western Blot ved hjælp af immunoreaktionsfremmende teknologi

DOI:
10.3791/61657-v

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05:59 min

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September 26, 2020

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, , , , ,
1Laboratory of Developmental Neurobiology, Cell and Developmental Biology Center, National Heart, Lung, and Blood Institute,National Institutes of Health, 2United Graduate School of Drug Discovery and Medical Information Sciences,Gifu University

Chapters

  • 00:05Introduction
  • 00:51Incubation with Primary Antibody
  • 01:59Membrane Wash
  • 02:59Image Acquisition
  • 03:52Results: Optimization and Validation of CDR Method in conjunction with IRE
  • 05:22Conclusion

Summary

Automatic Translation

Automatic Translation

En ultra-high-speed vestlige blotting teknik er udviklet ved at forbedre kinetik af antigen-antistof bindende gennem cyklisk dræning og genopfyldning (CDR) teknologi i forbindelse med en immunoreaktion styrke agent.

Tags

Ultra-high-speed Western BlotImmunoreaction Enhancing TechnologyPVDF MembranePrimary AntibodySecondary AntibodyChemiluminescent SubstrateSalad SpinnerCyclic Draining And ReplenishingBiomedical ResearchTime-saving

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