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Successful In vivo Calcium Imaging with a Head-Mount Miniaturized Microscope in the Amygdala of Freely Behaving Mouse
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Neuroscience
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JoVE Journal Neuroscience
Successful In vivo Calcium Imaging with a Head-Mount Miniaturized Microscope in the Amygdala of Freely Behaving Mouse

Successful In vivo Calcium Imaging with a Head-Mount Miniaturized Microscope in the Amygdala of Freely Behaving Mouse

DOI:
10.3791/61659-v

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09:39 min

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August 26, 2020

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,
1Department of Biological Sciences,Korea Advanced Institute of Science and Technology (KAIST), 2KAIST Institute for BioCentury (KIB),Korea Advanced Institute of Science and Technology (KAIST)

Chapters

  • 00:05Introduction
  • 00:41Adeno-Associated Virus (AAV) Injection
  • 02:23Gradient Refractive Index (GRIN) Lens Implantation
  • 06:11GRIN Lens Implantation Validation
  • 07:11Results: Representative GRIN Lens Implantation Validation and GCaMP Signal Evaluation
  • 08:58Conclusion

Summary

Automatic Translation

Automatic Translation

In vivo microendoscopic calcium imaging is an invaluable tool that enables real-time monitoring of neuronal activities in freely behaving animals. However, applying this technique to the amygdala has been difficult. This protocol aims to provide a useful guideline for successfully targeting amygdala cells with a miniaturized microscope in mice.

Tags

In Vivo Calcium ImagingMiniaturized MicroscopyAmygdalaAAV InjectionStereotaxic FrameCraniotomyViral InjectionSkull ScrewsMotorized Surgery ArmStereotactic FrameAmygdala Coordinates

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