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Journal
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Bioengineering
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心肌梗塞穆林模型中的 3D 生物打印贴片移植
JoVE Journal
Bioengineering
Author Produced
This content is Free Access.
JoVE Journal
Bioengineering
Transplantation of a 3D Bioprinted Patch in a Murine Model of Myocardial Infarction
Please note that all translations are automatically generated.
Click here for the English version.
心肌梗塞穆林模型中的 3D 生物打印贴片移植
DOI:
10.3791/61675-v
•
07:47 min
•
September 26, 2020
•
Christopher D. Roche
2,3,4
,
Carmine Gentile
2,3
1
The University of Sydney
,
2
University of Technology Sydney (UTS)
,
3
The Royal North Shore Hospital
,
4
University Hospital of Wales
Chapters
00:00
Introduction
00:42
Surgical Procedure
01:18
Preparing the Surgical Field
01:31
Left Lateral Thoracotomy
02:00
Left Anterior Descending (LAD) Permanent Coronary Artery Ligation
02:58
3D Bioprinting and Culturing Patches (7-14 days before transplantation)
04:19
Transplantation of the Bioprinted Patch onto the Epicardium
05:39
Mouse Recovery
06:29
Representative Results
06:45
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议旨在移植一个3D生物打印补丁到梗死小鼠的史诗建模心力衰竭。它包括有关麻醉、手术胸腔开口、左前降(LAD)冠状动脉的永久结扎以及将生物打印的贴片应用到心脏梗塞区域的详细信息。
Tags
3D Bioprinting
Stem Cell
Heart Patch
Myocardial Infarction
Cardiac Cells
Hydrogel
LAD Ligation
Mouse Model
Transplantation
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