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Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing
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Neuroscience
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JoVE Journal Neuroscience
Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing

Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing

DOI:
10.3791/61742-v

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07:28 min

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January 06, 2021

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, , , , , , , ,
1Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences

Chapters

  • 00:04Introduction
  • 00:45Innervation of Rat Cranial Dura Mater
  • 02:34Retrograde Tracing Study with Fluorogold
  • 05:20Results: Neurovascular Structure of the Cranial Dura Mater and Retrograde-labeled Neurons in the TG and DRGs
  • 07:02Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol to visualize spatial correlation of calcitonin gene-related peptide (CGRP)-immunoreactive nerve fibers and blood vessels in the cranial dura mater using immunofluorescence and fluorescent histochemistry with CGRP and phalloidin, respectively. In addition, the origin of these nerve fibers was retrograde traced with a fluorescent neural tracer.

Tags

Keywords: CGRPDura MaterImmunofluorescenceNeural TracingCranial NervesBlood VesselsRatConfocal MicroscopyWhole MountImmunohistochemistryFluorogold

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