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Intravitreal injektion og kvantisering af infektionsparametre i en musemodel af bakteriel endophthalmitis
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Immunology and Infection
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JoVE Journal Immunology and Infection
Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis

Intravitreal injektion og kvantisering af infektionsparametre i en musemodel af bakteriel endophthalmitis

DOI:
10.3791/61749-v

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07:24 min

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February 06, 2021

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, , , , ,
1Department of Microbiology and Immunology,University of Oklahoma Health Sciences Center, 2Department of Ophthalmology,Dean McGee Eye Institute, 3Dean McGee Eye Institute, 4Department of Cell Biology and Department of Family and Preventive Medicine,University of Oklahoma Health Sciences Center

Chapters

  • 00:04Introduction
  • 00:54Bacillus cereus Culture and Dilution
  • 01:48Mouse Intravitreal Injection
  • 04:02Eye Harvest
  • 04:43Intraocular Bacterial Count
  • 06:06Results: Representative Bacterial Endophthalmitis Mouse Model Validation
  • 06:47Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver her en metode til intravitreal injektion og efterfølgende bakteriel kvælning i musemodel af bakteriel endophthalmitis. Denne protokol kan udvides til måling af værtens immunrespons og bakterielle og værtsgenetielle udtryk.

Tags

Intravitreal InjectionMouse ModelBacterial EndophthalmitisPathogenesisIntraocular InfectionQuantitationMicroorganismsHost Immune ResponsesGene ExpressionTreatment EffectivenessBiosafety Level TwoB. CereusMicroinjectorOphthalmic MicroscopeAnesthetized MouseReverse Action Forceps

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