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Højdimensionalitetsflowcytometri til immunfunktionsanalyse af dissekeret implantatvæv
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues
DOI:

08:21 min

September 15, 2021

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Chapters

  • 00:05Introduction
  • 00:40Cell Isolation
  • 02:50Cell Surface Marker Staining
  • 04:27Cytometer and Unmixing Setup
  • 05:37Results: Representative 14-Color FACS Staining on Mouse Tissue
  • 07:25Conclusion

Summary

Automatic Translation

Isolering af celler fra dissekerede implantater og deres karakterisering ved flowcytometri kan bidrage væsentligt til at forstå mønsteret af immunrespons mod implantater. Dette papir beskriver en præcis metode til isolering af celler fra dissekerede implantater og deres farvning til flowcytometrisk analyse.

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