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Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia
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Cancer Research
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JoVE Journal Cancer Research
Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia

Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia

DOI:
10.3791/61803-v

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05:24 min

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February 21, 2021

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, , , , , ,
1Department of Biomedicine,University Hospital Basel and University of Basel, 2Division for Hematology,University Hospital Basel and University of Basel, 3Department of Internal Medicine, Hematology and Oncology,University Hospital Tübingen

Chapters

  • 00:04Introduction
  • 00:43Biotinylation of the NKG2D Fusion Protein and Thawing of Primary AML Cells
  • 02:00Staining of Primary AML Cells with the Biotinylated NKG2D Fusion Protein
  • 03:24Results: Gating Strategy and Staining Protocol Comparison
  • 04:49Conclusion

Summary

Automatic Translation

Automatic Translation

We present two different staining protocols for NKG2D ligand (NKG2DL) detection in human primary acute myeloid leukemia (AML) samples. The first approach is based on a fusion protein, able to recognize all known and potentially yet unknown ligands, while the second protocol relies on the addition of multiple anti-NKG2DL antibodies.

Tags

Keywords: NKG2D LigandsFlow CytometryAcute Myeloid LeukemiaLeukemic Stem CellsCell Surface DetectionBiotinylated NKG2D Fusion ProteinStreptavidin PE

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