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Journal
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Bioengineering
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在机械生物学研究的轨道摇床上的 6 井板中分段内皮细胞的生长
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Bioengineering
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Bioengineering
Segmenting Growth of Endothelial Cells in 6-Well Plates on an Orbital Shaker for Mechanobiological Studies
Please note that all translations are automatically generated.
Click here for the English version.
在机械生物学研究的轨道摇床上的 6 井板中分段内皮细胞的生长
DOI:
10.3791/61817-v
•
12:28 min
•
June 03, 2021
•
Kuin T. Pang*
1,2
,
Mean Ghim*
1
,
Mehwish Arshad
,
Xiaomeng Wang
3,4
,
Peter D. Weinberg
1
Department of Bioengineering
,
Imperial College London
,
2
Institute of Molecular and Cell Biology
,
Agency for Science, Technology and Research (A*STAR)
,
3
Lee Kong Chian School of Medicine
,
Nanyang Technological University Singapore
,
4
Singapore Eye Research Institute
,
The Academia
Chapters
00:00
Introduction
01:33
Fabrication of Stainless-steel Module
01:43
3D Printing of Polydimethylsiloxane (PDMS) Mold
02:39
Casting of PDMS Ring
03:41
Preparation of 1% Pluronic F-127
04:32
Coating of a 6-well Plate
08:20
Seeding of Endothelial Cells
08:58
Shear Stress Application using an Orbital Shaker
09:57
Representative Results
11:31
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了一种涂层方法,使用轨道摇床模型将内皮细胞生长限制在 6 井板的特定区域,用于剪切应力应用。
Tags
Keywords: Endothelial Cells
6-well Plates
Orbital Shaker
Mechanobiology
Shear Stress
Cell Growth Segmentation
PDMS Ring
Pluronic F-127
3D Printing
Coating Method
Cell Culture
Atherosclerosis
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