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Decellularization of the Murine Cardiopulmonary Complex
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Cancer Research
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JoVE Journal Cancer Research
Decellularization of the Murine Cardiopulmonary Complex

Decellularization of the Murine Cardiopulmonary Complex

DOI:
10.3791/61854-v

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08:34 min

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May 30, 2021

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, , , , ,
1Biotech Research and Innovation Centre (BRIC),University of Copenhagen (UCPH), 2Department of Laboratory Medicine, Division of Translational Cancer Research,Lund University

Chapters

  • 00:04Introduction
  • 00:50Post-Mortem Microsurgery
  • 03:26Decellularization and Immunostaining
  • 05:30Hematoxylin-eosin Staining
  • 07:08Results: Multiple Protein Immunostaining of Decellularized Mouse Lungs
  • 07:48Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol aims to decellularize the heart and lungs of mice. The resulting extracellular matrix (ECM) scaffolds can be immunostained and imaged to map the location and topology of their components.

Tags

DecellularizationMurineCardiopulmonary ComplexExtracellular MatrixStructural IntegrityBiochemical AnalysisAnatomical AnalysisThoracic WallSternotomyThymusBrachiocephalic ArteriesTracheaBronchiDescending Aorta

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