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Laserfri Hydroxyl Radical Protein Footprinting til at udføre højere orden strukturel analyse af proteiner
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Biochemistry
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JoVE Journal Biochemistry
Laser-free Hydroxyl Radical Protein Footprinting to Perform Higher Order Structural Analysis of Proteins

Laserfri Hydroxyl Radical Protein Footprinting til at udføre højere orden strukturel analyse af proteiner

DOI:
10.3791/61861-v

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09:59 min

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June 04, 2021

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, , ,
1GenNext Technologies Inc., 2Department of Biomolecular Sciences, School of Pharmacy,University of Mississippi, 3Department of Chemistry and Biochemistry,University of Mississippi

Chapters

  • 00:04Introduction
  • 00:35Installing the Capillary Tube
  • 02:43Installing an Injection Loop
  • 03:34Initializing the Laser Free HRPF System
  • 04:45Modification of Protein to Detect Changes in Higher Order Structure
  • 06:59Shutting Down the Laser Free HRPF System
  • 07:43Results: High Order Structural Analysis of Proteins Using Laser-free Hydroxyl Radical Protein Footprinting
  • 09:15Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol præsenterer en metode til at bruge inline radikal dosimetri og en plasmalyskilde til at udføre flash oxidationsproteinfodaftryk. Denne metode erstatter den farlige UV-laser for at forenkle og forbedre reproducerbarheden af hurtig fotokemisk oxidation af proteinundersøgelser.

Tags

Protein FootprintingHydroxyl RadicalLaser-freeProtein StructureProtein InteractionProtein AggregationProtein StabilityCapillaryPhotolysisDosimetrySilicaPolyimideNutFerrulePhotolysis CellDosimeter CellMagnetic MaskTeflon TubingInjection Loop

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