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从小鼠中分离心肌细胞的安特格拉德灌注方法
JoVE Journal
Biology
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JoVE Journal Biology
An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice
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从小鼠中分离心肌细胞的安特格拉德灌注方法

DOI:
10.3791/61866-v

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08:33 min

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May 19, 2021

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, , ,
1Department of Physiology,Shiga University of Medical Science

Chapters

  • 00:04Introduciton
  • 00:48Mouse Heart Excision and Aorta Preparation
  • 02:35Antegrade Mouse Heart Perfusion
  • 04:26Individual Heart Cell Isolation
  • 06:37Results: Representative Isolated Cardiomyocyte Imaging and Analysis
  • 07:52Conclusion

Summary

Automatic Translation

Automatic Translation

我们开发了一种通过前代灌注技术分离高质量个体小鼠心脏细胞的简化e方法 。这种方法是无兰根多夫和有用的隔离心室和心室肌细胞或间质细胞，如心脏成纤维细胞或祖先。

Tags

Mouse Cardiomyocyte IsolationAntegrade PerfusionLangendorff-freeHeart Cell IsolationCardiac Myocyte IsolationCIB-EGTAPerfusion PlateInfusion PumpVascular Clamp

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