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Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad
DOI:

09:08 min

October 17, 2020

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Chapters

  • 00:05Introduction
  • 00:46Day-of-Dissection Preparation
  • 02:35Dissection
  • 04:53Imaging
  • 06:46Results: The Live-Imaging Results of the Cultured Gonads, Their Health, and Niche Compaction
  • 08:15Conclusion

Summary

Automatic Translation

Here, we provide a dissection protocol required to live-image the late embryonic Drosophila male gonad. This protocol will permit observation of dynamic cellular processes under normal conditions or after transgenic or pharmacological manipulation.

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