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Journal / Bioengineering / Building a Simple and Versatile Illumination…
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Bioengineering

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JoVE Journal Bioengineering
Building a Simple and Versatile Illumination System for Optogenetic Experiments
 

Building a Simple and Versatile Illumination System for Optogenetic Experiments

Article DOI: 10.3791/61914-v • 06:41 min • January 12th, 2021
January 12th, 2021 •
1, 2, 1, 1, 1, 3, 1, 1, 4, 1, 1
1Department of Bioengineering, University of California, San Diego, 2University of California, San Diego, 3Department of Mathematical Computational, and Systems biology, University of California, Irvine, 4Department of Biomedical Engineering, Duke University

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Summary
Automatic Translation

This protocol describes how to perform optogenetic experiments for controlling gene expression with red and far-red light using PhyB and PIF3. Included are step-by-step instructions for building a simple and flexible illumination system, which enables the control of gene expression or other optogenetics with a computer.

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Keywords: Illumination System Optogenetic Experiments LED Electrical Circuit Arduino Soldering Wire Connector Resistor Cathode Anode Solder Paste Shrink Tube Flux
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