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Immunology and Infection

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由加德纳菌阴道膀胱暴露在小鼠中引发的复发性大肠杆菌尿路感染

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice

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由加德纳菌阴道膀胱暴露在小鼠中引发的复发性大肠杆菌尿路感染

DOI:

10.3791/61967-v

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08:53 min

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December 04, 2020

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Valerie P. O’Brien^2,6, Matthew S. Joens⁷, Amanda L. Lewis^2,4,8, Nicole M. Gilbert⁵

¹Department of Molecular Microbiology,Washington University School of Medicine in Saint Louis, ²Center for Women’s Infectious Disease Research,Washington University School of Medicine in Saint Louis, ³Center for Cellular Imaging,Washington University School of Medicine in Saint Louis, ⁴Department of Obstetrics and Gynecology,Washington University School of Medicine in Saint Louis, ⁵Department of Pediatrics,Washington University School of Medicine in Saint Louis, ⁶Fred Hutchinson Cancer Research Center, ⁷TESCAN USA, Inc., ⁸University of California San Diego

Chapters

00:05Introduction
00:41Bladder Exposures to G. vaginalis
01:53Urine Cytology
02:31Imaging Bladders by Scanning Electron Microscopy
06:23Results: G. vaginalis Triggers UPEC rUTI
08:27Conclusion

Summary

Automatic Translation

展示了产尿道 大肠杆菌 （UPEC）经尿道接种以建立潜伏细胞内膀胱储液囊的小鼠模型，随后膀胱暴露于 阴道 G. 以诱导复发性 UPEC UTI。还证明了细菌的计数，尿液细胞学检查以及原位膀胱固定和扫描电子显微镜处理。

Tags

Keywords: Recurrent UTI Escherichia Coli Gardnerella Vaginalis Bladder Exposure Mouse Model Urogenital Bacteria Bladder Exfoliation Anaerobic Culture Bacterial Inoculum Urine Analysis Cytospin PMNs Epithelial Cells Catheterization Bladder Exposure

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