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Journal / Biochemistry / Complementation of Splicing Activity by a…
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Biochemistry

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JoVE Journal Biochemistry
Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads
 

Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads

Article DOI: 10.3791/61990-v • 08:48 min • December 9th, 2020
December 9th, 2020 •
1, 1, 2, 1
1Department of Biochemistry and Molecular Biology, Michigan State University, 2Department of Microbiology and Molecular Genetics, Michigan State University

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Summary
Automatic Translation

This article describes the experimental procedures for (a) depletion of U1 snRNP from nuclear extracts, with concomitant loss of splicing activity; and (b) reconstitution of splicing activity in the U1-depleted extract by galectin-3 - U1 snRNP particles bound to beads covalently coupled with anti-galectin-3 antibodies.

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Keywords: Galectin-3 U1 SnRNP Pre-mRNA Splicing Immunoselection Glycerol Gradient Nuclear Extract Anti-U1 Beads Dialysis Anti-gal3 Beads
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