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1Center for Gene Therapy,The Research Institute at Nationwide Children’s Hospital, 2Center for Cardiovascular Research,The Research Institute at Nationwide Children’s Hospital, 3The Heart Center,Nationwide Children’s Hospital, 4Division of Genetic and Genomic Medicine,Nationwide Children’s Hospital, 5Department of Human Genetics,The University of Utah School of Medicine, 6Department of Pediatrics,The Ohio State University
Chapters
- 00:00Introduction
- 00:36Overview
- 01:23Initiation of Demal Fibroblasts Culture
- 02:46Establishment of FM Cell Lines Lentvirus-hTERT Transduction
- 03:57Lentivirus-MyoD Transduction
- 04:54Fibroblasts Transdifferentiation
- 06:11Results
- 09:16Conclusion
Este protocolo describe la conversión de fibroblastos de piel en mioblastos y su diferenciación en miotubes. Las líneas celulares se derivan de pacientes con trastornos neuromusculares y se pueden utilizar para investigar mecanismos patológicos y para probar estrategias terapéuticas.
