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增强分子化合物输送到细胞的声透流体装置的组装和操作

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Assembly and Operation of an Acoustofluidic Device for Enhanced Delivery of Molecular Compounds to Cells

Please note that all translations are automatically generated. Click here for the English version.

增强分子化合物输送到细胞的声透流体装置的组装和操作

DOI:

10.3791/62035-v

•

07:16 min

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January 21, 2021

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Connor S. Centner, Emily M. Murphy, Bryce F. Stamp, Mariah C. Priddy, John T. Moore, Paula J. Bates, Michael A. Menze, Kavitha Yaddanapudi, Jonathan A. Kopechek

¹Department of Bioengineering,University of Louisville, ²School of Medicine,University of Louisville, ³Department of Biology,University of Louisville

Chapters

00:04Introduction
00:45Fabrication of Acoustofluidic Device
02:18Assembly and Operation of Acoustofluidic System
04:08Preparation of Ultrasound Contrast Agents
05:38Results: Acoustofluidic Treatment Enhances Intracellular Molecular Delivery
06:30Conclusion

Summary

Automatic Translation

该协议描述了低成本声学流体装置的组装和运行，该装置通过超声波对比剂诱导的声波快速分子输送到细胞。

Tags

Acoustofluidic Device Biomolecule Delivery Cell Transformation PDMS Photoresist Wafer Piezo Transducer Microcontroller 8 MHz Signal Tygon Tubing

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