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网站特定赖氨酸乙酰化的组蛋白为核体重组使用遗传密码扩展在 埃斯切里西亚大肠杆菌

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Biochemistry

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JoVE Journal Biochemistry

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli

Please note that all translations are automatically generated. Click here for the English version.

网站特定赖氨酸乙酰化的组蛋白为核体重组使用遗传密码扩展在埃斯切里西亚大肠杆菌

DOI:

10.3791/62113-v

•

07:26 min

•

December 26, 2020

•

Chesley Marie Rowlett, Wenshe Ray Liu

¹Department of Chemistry,Texas A&M University

Chapters

00:04Introduction
00:34Acetylated Histone Protein Expression
03:13Assembly of Histone Octamer
04:34Nucleosome Assembly
06:04Results: Histone Dimers, Tetramers, Octamers, and Assembled his Tagged and TEV Digested Nucleosomes
06:45Conclusion

Summary

Automatic Translation

在这里，我们提出一种方法来表达使用遗传密码膨胀和组装重组核体体外的自负型高石蛋白。

Tags

Lysine Acetylation Histones Nucleosome Reconstitution Genetic Code Expansion Escherichia Coli Mononucleosome Epigenetic Experiments Biochemical Assays Binding Assays Structure Determination Histone Lysis Buffer Inclusion Bodies Nickel-NTA Purification Acetylated Histone Protein Dialysis Acetic Acid Buffer

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