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Journal / Biochemistry / Cellefraktionering af U937-celler ved isopycnic…
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Biochemistry

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JoVE Journal Biochemistry
Cellefraktionering af U937-celler ved isopycnic densitetsgradientrensning
 
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Cellefraktionering af U937-celler ved isopycnic densitetsgradientrensning

Article DOI: 10.3791/62119-v • 10:19 min • August 12th, 2021
August 12th, 2021 •
1, 1, 1, 1, 1
1Department of Basic and Clinical Sciences, Albany College of Pharmacy and Health Sciences

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Denne fraktioneringsprotokol giver forskere mulighed for at isolere cytoplasmatiske, nukleare, mitokondrie- og membranproteiner fra pattedyrceller. De to sidstnævnte subcellulære fraktioner renses yderligere via isopycnic densitetsgradient.

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Biokemi udgave 174 cellulær fraktionering nuklear mitokondrier membran cytoplasma organeller ultracentrifugering densitetsgradient U937
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