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Establecimiento de un sistema de cultivo de esferoides epidérmicos de alto rendimiento para modelar la plasticidad de las células madre de queratinocitos
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1Department of Pathology, Microbiology and Immunology,University of South Carolina School of Medicine, 2Department of Dermatology,Brigham and Women’s Hospital, Harvard Medical School, 3Department of Biochemistry and Molecular Biology,University of Maryland School of Medicine Baltimore, 4Department of Pathology,Albert Einstein College of Medicine, 5Department of Pediatrics,Vanderbilt University Medical Center, 6Department of Drug Discovery and Biomedical Sciences, College of Pharmacy,University of South Carolina
Chapters
- 00:04Introduction
- 00:27Isolation and Culture of Human Keratinocytes from Neonatal Foreskin Tissue
- 02:37Generating Skin Epidermosphere Cultures in Vitro
- 05:02Epidermal Spheroid Re-Plating Assay
- 05:42Characterization of Spheroid-Derived (SD) Sub-Populations by FACS
- 08:06Results: Characterization of Epidermal Spheroid Formation and Spheroid-Derived NHKs
- 09:30Conclusion
Aquí describimos un protocolo para el cultivo sistemático de esferoides epidérmicos en cultivo de suspensión 3D. Este protocolo tiene una amplia gama de aplicaciones para su uso en una variedad de tipos de tejidos epiteliales y para el modelado de varias enfermedades y afecciones humanas.
