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Análisis de síntesis de lípidos neutros en Saccharomyces cerevisiae mediante etiquetado metabólico y cromatografía en capa delgada
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography
DOI:

08:30 min

February 02, 2021

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Chapters

  • 00:04Introduction
  • 00:30Growth and Labelling of Yeast Cells
  • 02:55Isolation of Total Lipids from Yeast
  • 04:42Thin Layer Chromatography and Quantification of Results
  • 07:09Results: 14C-Acetic Acid Radiolabeling Detection Analysis of Multiple NL Species
  • 08:03Conclusion

Summary

Automatic Translation

Aquí, se presenta un protocolo para el etiquetado metabólico de levaduras con ácido 14C-acético, que se combina con cromatografía de capa delgada para la separación de lípidos neutros.

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