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从预成型微气泡生产膜过滤相移十氟丁烷纳米滴
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Production of Membrane-Filtered Phase-Shift Decafluorobutane Nanodroplets from Preformed Microbubbles
DOI:

07:10 min

March 23, 2021

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Chapters

  • 00:04Introduction
  • 00:46Generating Microbubbles from Lipid Films
  • 01:48Preparing and Priming the Extruder for Microbubble Condensation
  • 03:13Pre-Cooling Microbubbles for Extrusion and Separating Droplets from Liposomes by Centrifugation
  • 05:04Results: Phase-Shift Droplet Analysis of DFB and OFP Nanodroplets Using DLS, TRPS and Microscopy
  • 06:19Conclusion

Summary

Automatic Translation

该协议描述了一种使用探针尖端超声处理产生大量脂质封装的十氟丁烷微气泡的方法,然后使用高压挤出和机械过滤将它们冷凝成相移纳米滴。

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