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Bioengineering
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从预成型微气泡生产膜过滤相移十氟丁烷纳米滴
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Bioengineering
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JoVE Journal
Bioengineering
Production of Membrane-Filtered Phase-Shift Decafluorobutane Nanodroplets from Preformed Microbubbles
Please note that all translations are automatically generated.
Click here for the English version.
从预成型微气泡生产膜过滤相移十氟丁烷纳米滴
DOI:
10.3791/62203-v
•
07:10 min
•
March 23, 2021
•
Darrah A. Merillat
,
Arvin Honari
,
Shashank R. Sirsi
2
1
Department of Bioengineering
,
The University of Texas at Dallas
,
2
Department of Radiology
,
The University of Texas Southwestern
Chapters
00:04
Introduction
00:46
Generating Microbubbles from Lipid Films
01:48
Preparing and Priming the Extruder for Microbubble Condensation
03:13
Pre-Cooling Microbubbles for Extrusion and Separating Droplets from Liposomes by Centrifugation
05:04
Results: Phase-Shift Droplet Analysis of DFB and OFP Nanodroplets Using DLS, TRPS and Microscopy
06:19
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了一种使用探针尖端超声处理产生大量脂质封装的十氟丁烷微气泡的方法,然后使用高压挤出和机械过滤将它们冷凝成相移纳米滴。
Tags
Membrane-filtered Phase-shift Decafluorobutane Nanodroplets
Preformed Microbubbles
Biomedical Applications
Lipid Microbubbles
Gas Microbubbles
200-nanometer Ceramic Filter
High-pressure Extruder
Nitrogen Gas Tank
PBS
Polydispersity Reduction
Size Control
Cost-effective Method
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