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Tumor Allotransplantation in Drosophila melanogaster with a Programmable Auto-Nanoliter Injector
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Cancer Research
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JoVE Journal Cancer Research
Tumor Allotransplantation in Drosophila melanogaster with a Programmable Auto-Nanoliter Injector

Tumor Allotransplantation in Drosophila melanogaster with a Programmable Auto-Nanoliter Injector

DOI:
10.3791/62229-v

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05:06 min

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February 02, 2021

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, , , , , ,
1Department of Biochemistry and Molecular Biology,Tulane University School of Medicine

Chapters

  • 00:05Introduction
  • 00:39Preparation of Adult Wild Type Drosophila for Allotransplantation and Assembly of Autoinjector Apparatus
  • 02:20Allograft of Primary SG Imaginal Ring Tumor and Re-Allograft of Transplanted Tumors
  • 03:52Results: Fluorescence Imaging Analysis to Locate Appropriate Tumors in Host Abdomen
  • 04:27Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol provides detailed guidance for the initial and continued generational allotransplantation of Drosophila tumors into the abdomen of adult hosts for studying various aspects of neoplasia. Using an autoinjector apparatus, researchers can achieve improved efficiency and tumor yields compared to those achieved by traditional, manual methods.

Tags

Tumor AllotransplantationDrosophila MelanogasterAutoinjectorSchneider’s MediumSG Imaginal Ring TumorMicro Pipette PullerGlass CapillaryTumor DissectionCancer Research

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