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颗粒模板化乳化可实现无微流体液滴检测
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Particle Templated Emulsification enables Microfluidic-Free Droplet Assays
Please note that all translations are automatically generated.
Click here for the English version.
颗粒模板化乳化可实现无微流体液滴检测
DOI:
10.3791/62248-v
•
11:03 min
•
March 09, 2021
•
Daniel W. Weisgerber
,
Makiko N. Hatori
,
Adam R. Abate
2
1
Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences
,
University of California
,
2
Chan Zuckerberg Biohub
Chapters
00:04
Introduction
00:44
Preparation of Hydrogel Particles for Particle Templated Emulsification
02:31
Fabrication of the Custom Microfluidic Device
04:37
Fabrication of Templating Particles
06:26
Particle Templated Emulsification
08:26
Digital Droplet PCR and Analysis
09:02
Results: Sample Encapsulation Using Particle Templated Emulsification (PTE)
10:30
Conclusion
Summary
Automatic Translation
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Automatic Translation
油包水液滴测定可用于分析化学、酶进化和单细胞分析,但通常需要微流体才能形成液滴。在这里,我们描述了颗粒模板化乳化,这是一种进行液滴测定的无微流体方法。
Tags
Particle Templated Emulsification
Microfluidic-free
Droplet Assays
Digital Droplet PCR
Emulsification
Polyacrylamide Particles
Photoresist
PDMS
Microfluidic Fabrication
Photomask
Post Exposure Baking
PGMEA
Isopropanol
Plasma Treatment
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