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Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton
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Biology
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JoVE Journal Biology
Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton

Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton

DOI:
10.3791/62271-v

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08:59 min

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February 25, 2021

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, , , ,
1The National Resource for Automated Molecular Microscopy,Simons Electron Microscopy Center, New York Structural Biology Center, 2piTree Software

Chapters

  • 00:04Introduction
  • 00:49Set Up the Spotiton Machine and Software
  • 03:40Prepare Two-sample, Mixed Grids
  • 07:09Results: System Camera Images and the Pattern of Ice Deposition on the Spotiton-prepared Grid
  • 08:16Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol presented here describes the use of Spotiton, a novel robotic system, to deliver two samples of interest onto a self-wicking, nanowire grid that mix for a minimum of 90 ms prior to vitrification in liquid cryogen.

Tags

Cryo-electron MicroscopySpotitonRobotic SystemTime-resolved StudiesProtein ConformationMembrane Channel ActivationDNA/RNA SynthesisDrug/antibody InteractionGrid PreparationNitrogen SupplyWater ReservoirRobot InitializationTip PrimingDroplet InspectionGrid LoadingPlunge Path Camera

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