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Chapters
- 00:04Introduction
- 00:32Filtration Procedure for Seawater Dissolved and Particulate Lipids
- 01:53Liquid-Liquid Extraction of Seawater or Liquid Samples
- 03:23Extraction Protocol for Solids (Modified Folch et al. Extraction)
- 06:17Developing and Conditioning Sequences for Separation of Aquatic Lipid Classes by Rod TLC
- 10:11FAME Derivatization with H2SO4 in MeOH
- 13:17Representative Results
- 14:30Conclusion
Denne protokol er til bestemmelse af lipider i havvand og biologiske prøver. Lipider i filtrater ekstraheres med chloroform eller blandinger af chloroform og methanol i tilfælde af faste stoffer. Lipid klasser måles ved stang tynd-lag kromatografi med flamme ionisering afsløring og deres sum giver det samlede lipid indhold.
