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Human Ex vivo Sår Model og Whole-Mount farvning tilgang til præcist evaluere hudreparation
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair

Human Ex vivo Sår Model og Whole-Mount farvning tilgang til præcist evaluere hudreparation

DOI:
10.3791/62326-v

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07:32 min

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February 17, 2021

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, , ,
1Centre for Atherothrombosis and Metabolic Disease, Hull York Medical School,University of Hull

Chapters

  • 00:05Introduction
  • 00:39Preparation of Skin for Wounding and Creating Ex Vivo Human Skin Wounds
  • 02:36Whole Mount Staining of Ex Vivo Wounds
  • 04:14Explant Imaging
  • 05:18Results: Wound Closure Evaluation by Whole-Mount Tissue Staining
  • 07:06Conclusion

Summary

Automatic Translation

Automatic Translation

Her demonstrerer vi en optimeret teknik til vurdering af sårreparation ved hjælp af ex vivo menneskelig hud kombineret med en helmonteret farvningsmetode. Denne metode giver en præklinisk platform til vurdering af potentielle sårbehandlinger.

Tags

Ex VivoHuman SkinWound ModelWhole-mount StainingSkin RepairWound AssessmentSkin ExplantWound HealingSkin FixativeFluorescent Staining

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