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Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis

Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis

DOI:
10.3791/62373-v

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12:08 min

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June 19, 2021

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, ,
1Champalimaud Centre for the Unknown, Champalimaud Foundation, 2Instituto Gulbenkian de Ciência

Chapters

  • 00:00Introduction
  • 01:37Setting Up for Injection
  • 02:0424 Hours Before Injection
  • 02:48Injection Day
  • 04:24Injection Procedures
  • 06:12Metastasic Assay
  • 07:104 Day Post-Injection
  • 08:05Whole Mount Immunostining for Confocal Imaging
  • 08:35Mounting of Xenografts
  • 09:02Confocal Imaging
  • 10:22Representative Results
  • 11:45Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we provide a step-by-step protocol, with tips to generate xenografts and guidelines for tumor behavior analysis, whole-mount immunofluorescence, and confocal imaging quantification.

Tags

Keywords: Zebrafish XenograftsCancer ResearchLarva ModelProliferationAngiogenesisMetastasisTumor MicroenvironmentAnti-cancer TherapiesPatient-derived CellsCell InjectionIn-vitro ConfluenceZebrafish EmbryosAgarose PlatesMicroinjection

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