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Harvesting and Disaggregation: An Overlooked Step in Biofilm Methods Research
JoVE Journal
Biology
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JoVE Journal Biology
Harvesting and Disaggregation: An Overlooked Step in Biofilm Methods Research
DOI:

13:25 min

April 22, 2022

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Chapters

  • 00:47Introduction
  • 01:53Vortexing and Sonication
  • 04:29Scraping and Homogenization
  • 07:31Scraping, Vortexing, and Sonication
  • 09:48Results
  • 12:16Conclusion

Summary

Automatic Translation

This paper details methods that demonstrate three common biofilm harvesting and disaggregation techniques on two surface types, ruggedness testing of a harvesting method and minimum information to consider when choosing and optimizing harvesting and disaggregation techniques to increase reproducibility.

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