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Overvåking av dynamisk vekst av retinale kar i oksygenindusert retinopati musemodell
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JoVE Journal Biology
Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model

Overvåking av dynamisk vekst av retinale kar i oksygenindusert retinopati musemodell

DOI:
10.3791/62410-v

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10:32 min

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April 02, 2021

•

,
1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center,Sun Yat-Sen University

Chapters

  • 00:05Introduction
  • 00:58Preparation of Retinal Whole Mounts and Immunofluorescence Staining
  • 05:36In Vivo Imaging with Fluorescein Fundus Angiography (FFA)
  • 07:02FAA Image Processing
  • 08:40Results: Vaso-Obliteration (VO) and Neovascularization (NV) in the Oxygen-Induced Retinopathy (OIR) Mouse Model
  • 10:06Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokollen beskriver en detaljert metode for fremstilling og immunfluorescensfarging av mus retinal flat mounts og analyse. Bruk av fluorescein fundus angiografi (FFA) for musunger og bildebehandling er også beskrevet i detalj.

Tags

Retinal VesselsOxygen-induced RetinopathyMouse ModelRetinal Whole MountingRetinal VasculatureRetinal SeparationEye Dissection

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