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JoVE Journal
Bioengineering

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Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity
 

Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity

Article DOI: 10.3791/62429-v 07:26 min March 31st, 2021
March 31st, 2021

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Summary

Bacterial vesicles play important roles in pathogenesis and have promising biotechnological applications. The heterogeneity of vesicles complicates analysis and use; therefore, a simple, reproducible method to separate varying sizes of vesicles is necessary. Here, we demonstrate the use of size exclusion chromatography to separate heterogeneous vesicles produced by Aggregatibacter actinomycetemcomitans.

Tags

Keywords: Size Exclusion Chromatography Bacterial Outer Membrane Vesicle Heterogeneity Separation Nucleic Acids Proteins Density Gradient Ultracentrifugation A. Actinomycetemcomitans Gel Filtration Medium Elution Buffer Column Packing Sample Loading Fraction Collection Column Cleaning
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