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Bruke fluorescerende fargestoff, Rhodamine B, for å studere parring konkurranseevne i mannlige Aedes aegypti mygg
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Using the Fluorescent Dye, Rhodamine B, to Study Mating Competitiveness in Male Aedes aegypti Mosquitoes
DOI:

07:10 min

May 07, 2021

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Chapters

  • 00:04Introduction
  • 00:41Preparation of Male and Female Mosquitoes
  • 01:11Preparation of 0.2% RhB Sucrose Solution and Feeding of Male Mosquitoes
  • 01:52Checking for RhB Fluorescence in Male Mosquitoes
  • 02:46Mating Competitiveness Assay
  • 05:36Results: Assessment of Mating Competitiveness in Male Mosquitoes
  • 06:27Conclusion

Summary

Automatic Translation

Her presenterer vi en protokoll for å studere parring av konkurranseevnen til mannlige Aedes aegypti ved hjelp av fluorescerende fargestoff som markør. Kvinnelige mygg er utsatt for både merkede og umerkede menn for kopiering. Etter parring undersøkes spermathecae under et fluorescensmikroskop for å bestemme parringspartneren.

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