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Biology
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白细胞消除过滤器衍生的CD34 +细胞作为研究巨核细胞分化和血小板形成的细胞来源
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JoVE Journal
Biology
Leukodepletion Filters-Derived CD34+ Cells As a Cell Source to Study Megakaryocyte Differentiation and Platelet Formation
Please note that all translations are automatically generated.
Click here for the English version.
白细胞消除过滤器衍生的CD34 +细胞作为研究巨核细胞分化和血小板形成的细胞来源
DOI:
10.3791/62499-v
•
06:57 min
•
May 20, 2021
•
Anaïs Pongerard
,
Lea Mallo
,
Christian Gachet
,
Henri de La Salle
,
François Lanza
,
Catherine Strassel
1
BPPS UMR-S 1255, FMTS
,
Université de Strasbourg, INSERM, EFS Grand Est
Chapters
00:04
Introduction
01:01
Leukoreduction Filter (LRF) Back-Flushing Modality Preparation
01:48
PBMC Collection
02:47
CD34
+
Cell Selection
04:01
Evaluation of CD34
+
Cell Purity
04:35
CD34
+
Cell Freezing
05:07
Results: Mature Megakaryocyte Differentiation and Platelet Formation
06:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议详细描述了获得白细胞衍生的CD34 +造血祖细胞及其
体外
分化和成熟为能够释放培养基中血小板的含丙酸盐的巨核细胞所涉及的所有步骤。该程序可用于深入分析控制巨核细胞生成的细胞和分子机制。
Tags
Leukodepletion Filters
CD34+ Cells
Megakaryocyte Differentiation
Platelet Formation
Hematopoietic Progenitors
Megakaryopoiesis
PBMC Collection
Density Gradient Centrifugation
CD34+ Cell Selection
FC Receptor Blocking
CD34 Microbeads
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