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通过 FASP 消化和数据独立分析,EPS-尿液的蛋白质配置文件
JoVE Journal
Biochemistry
Author Produced
This content is Free Access.
JoVE Journal Biochemistry
Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
DOI:

14:48 min

May 08, 2021

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Chapters

  • 00:00Introduction
  • 00:55Protocol
  • 01:25Protocol: FASP Digestion
  • 05:29Protocol: SCX Purification
  • 08:08Protocol: Protein Quantifcation by External Standard
  • 09:23Protocol: NanoLC-MS/MS and DIA Analysis
  • 11:31Protocol: High-pH C18 Fractionation for Spectral Library
  • 13:09Protocol: Data Analysis
  • 13:35Expected Results
  • 13:53Conclusion

Summary

Automatic Translation

在这里,我们提出了一个优化的过滤消化协议,并提供了以下详细信息:蛋白质消化、肽纯化和数据独立获取分析。该策略应用于对表达的前列腺分泌物-尿液样本的分析,并允许高蛋白体覆盖率和低缺失值无标签尿素分析。

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