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Kleuring van de Cytoplasmatische Ca2+ met Fluo-4/AM in Appelpulp
JoVE Journal
Biology
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JoVE Journal Biology
Staining the Cytoplasmic Ca2+ with Fluo-4/AM in Apple Pulp
DOI:

08:05 min

November 06, 2021

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Chapters

  • 00:03Introduction
  • 00:57Protoplast Extraction
  • 01:49Small-molecule Calcium Ion Fluorescence Staining
  • 04:46Protoplast Viability Assay
  • 05:48Results
  • 07:21Conclusion

Summary

Automatic Translation

Geïsoleerde protoplasten van appelpulpcellen werden geladen met een calciumconsprecerend reagens om cytoplasmatische Ca2+ concentratie te detecteren.

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