Journal
/
Cancer Research
/
Tre metoder til differentialudtryksanalyse til RNA-sekventering: limma, EdgeR, DESeq2
JoVE Journal
Cancer Research
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Three Differential Expression Analysis Methods for RNA Sequencing: limma, EdgeR, DESeq2

Tre metoder til differentialudtryksanalyse til RNA-sekventering: limma, EdgeR, DESeq2

DOI:
10.3791/62528-v

•

10:10 min

•

September 18, 2021

•

, , , , ,
1Department of Obstetrics and Gynecology,Renmin Hospital of Wuhan University, 2Department of Pathology, Shanghai Skin Disease Hospital,Tongji University School of Medicine

Chapters

  • 00:08Introduction
  • 01:54I. Download and preprocess the data
  • 05:12II. RNA‐seq differential expression analysis through “limma”
  • 08:53III. RNA‐seq differential expression analysis through “edgeR”
  • 11:34IV. RNA‐seq differential expression analysis through “DESeq2”
  • 13:51V. Venn diagram
  • 14:36Conclusion

Summary

Automatic Translation

Automatic Translation

Der blev leveret en detaljeret protokol over differentialudtryksanalysemetoder til RNA-sekventering: limma, EdgeR, DESeq2.

Tags

RNA SequencingDifferential Expression AnalysisLimmaEdgeRDESeq2CholangiocarcinomaCancerGene ExpressionEnsemble Gene IDGene SymbolLow-expressed GenesDesign MatrixDGEListNormalizationLinear ModelT-statisticF-statisticLog-oddsLog2 Fold ChangeFDR

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62528?article_type=v

Brug RNA-sekventering til Detect Novel Splice Varianter Relateret til Drug Resistance i<em> In vitro</em> Kræft Modeller
check_url/62528?article_type=v

To-dimensionelle gelelektroforese kombineret med massespektrometri metoder til analyse af menneskelige hypofyseadenom væv proteom
check_url/62528?article_type=v

Tre-dimensionelle knogle ekstracellulære Matrix Model for osteosarkom
check_url/62528?article_type=v

Sequencing lille ikke-kodning RNA fra formalin-fast væv og serum-afledte Exosomer fra Kastrations resistente prostata cancer patienter
check_url/62528?article_type=v

Evaluering cell død ved hjælp af cellefri supernatant af probiotika i tre-dimensionelle sfæroid kulturer colorectal cancerceller
check_url/62528?article_type=v

En automatiseret differentiale nuklear farvning assay til nøjagtig bestemmelse af Mitocan Cytotoxicity
check_url/62528?article_type=v

Automatiseret dissektionsprotokol til tumorberigelse i væv med lavt tumorindhold
check_url/62528?article_type=v

Rumlig profilering af protein- og RNA-ekspression i væv: En tilgang til finjustering af virtuel mikrodissektion
check_url/62528?article_type=v

Fosfolipidmediator induceret transformation i tredimensionelle kulturer
check_url/62528?article_type=v

Transmitochondrial Cybrid Generation ved hjælp af kræftcellelinjer

Read Article