Journal
/
Medicine
/
Minimizing Post-Infusion Portal Vein Bleeding during Intrahepatic Islet Transplantation in Mice
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Minimizing Post-Infusion Portal Vein Bleeding during Intrahepatic Islet Transplantation in Mice

Minimizing Post-Infusion Portal Vein Bleeding during Intrahepatic Islet Transplantation in Mice

DOI:
10.3791/62530-v

•

06:41 min

•

May 10, 2021

•

, , ,
1Department of Surgery,Medical University of South Carolina, 2Georgetown University, 3Ralph H. Johnson Veterans Affairs Medical Center

Chapters

  • 00:04Introduction
  • 00:50Islet Preparation
  • 01:49Islet Transplantation Preparation
  • 02:32Gel Foam-Induced Hemostasis
  • 03:52Fat Pad-Induced Hemostasis
  • 04:38Results: Assessment of the Transplanted Islet Functionality
  • 05:55Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present refined surgical procedures on successfully performing intraportal islet transplantation, a clinically relevant but technically challenging surgical procedure, in mice.

Tags

Intrahepatic Islet TransplantationPortal Vein BleedingMouse ModelIslet CultureHBSSBSAInsulin SyringeAnesthetized MousePeritoneal CavityPortal VeinGel FoamBleeding Prevention

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation

3-Dimensional Resin Casting and Imaging of Mouse Portal Vein or Intrahepatic Bile Duct System

Direct Pressure Monitoring Accurately Predicts Pulmonary Vein Occlusion During Cryoballoon Ablation

Generation of Subcutaneous and Intrahepatic Human Hepatocellular Carcinoma Xenografts in Immunodeficient Mice

Heterotopic Auxiliary Rat Liver Transplantation With Flow-regulated Portal Vein Arterialization in Acute Hepatic Failure

Heterotopic Cervical Heart Transplantation in Mice

Using the Sleeve Technique in a Mouse Model of Aortic Transplantation – An Instructional Video

Rat Model of the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy (ALPPS) Procedure

Blood Collection Through Subclavian Vein Puncture in Mice

Fat-Covered Islet Transplantation Using Epididymal White Adipose Tissue

Read Article