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Billeddannelse og kvantificering af intakte neuronale dendritter via CLARITY Tissue Clearing
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Neuroscience
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JoVE Journal Neuroscience
Imaging and Quantification of Intact Neuronal Dendrites via CLARITY Tissue Clearing

Billeddannelse og kvantificering af intakte neuronale dendritter via CLARITY Tissue Clearing

DOI:
10.3791/62532-v

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07:45 min

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April 20, 2021

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, , , ,
1Department of Genetics and Genomics,Baylor College of Medicine, 2Medical Scientist Training Program,Baylor College of Medicine, 3Department of Neuroscience,Baylor College of Medicine

Chapters

  • 00:04Introduction
  • 00:45Tissue Clearing
  • 01:57Preparing and Mounting the Cleared Tissue
  • 04:19Imaging Cleared Tissue Samples
  • 06:07Results: Imaging and Quantification of Neuronal Dendrites
  • 07:22Conclusion

Summary

Automatic Translation

Automatic Translation

Neuronal dendritisk morfologi ligger ofte til grund for funktion. Faktisk manifesterer mange sygdomsprocesser, der påvirker udviklingen af neuroner, sig med en morfologisk fænotype. Denne protokol beskriver en enkel og kraftfuld metode til analyse af intakte dendritiske arbors og deres tilknyttede pigge.

Tags

Keywords: CLARITYNeuronal Dendrites3D ImagingNeuronal ConnectivitySpatial OrganizationElectrophoresisRefractive Index MatchingMicroscopy

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