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使用BrdU-DNA标记结合细胞周期鉴别成像评估全球DNA双链末端切除术

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Biology

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JoVE Journal Biology

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Please note that all translations are automatically generated. Click here for the English version.

使用BrdU-DNA标记结合细胞周期鉴别成像评估全球DNA双链末端切除术

DOI:

10.3791/62553-v

•

06:44 min

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April 28, 2021

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Julia O’Sullivan*^1,2, Sofiane Y. Mersaoui*^1,2, Guy Poirier³, Jean-Yves Masson²

¹Oncology Division,Genome Stability Laboratory, CHU de Québec Research Center, HDQ Pavilion, ²Department of Molecular Biology, Medical Biochemistry, and Pathology,Laval University Cancer Research Center, ³Oncology Division,CHU de Québec Research Center, CHUL Pavilion

Chapters

00:05Introduction
00:37Cell Culture, Treatments, and Coverslip Preparation
01:18Pre-Extraction and Fixation
02:13Permeabilization and Immunostaining
03:53Image Analysis
04:16Results: Visualization of DNA Double-Strand End Resection During S to G2 Phase of the Cell Cycle Using an Immunofluorescence-Based Method
06:05Conclusion

Summary

Automatic Translation

在本方案中，我们展示了如何使用基于免疫荧光的方法在细胞周期的S / G2阶段可视化DNA双链末端切除。

Tags

DNA Double-strand Break DNA Resection Cell Cycle BrdU Labeling Immunostaining Imaging

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