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珠子将蛋白质和核酸加载到附体人类细胞中
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Bead Loading Proteins and Nucleic Acids into Adherent Human Cells
DOI:

07:28 min

June 01, 2021

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Chapters

  • 00:04Introduction
  • 01:00Clean, Sterilize, and Dry Glass Beads
  • 02:26Assemble the Bead Loader Apparatus
  • 03:18Bead Loading Cells and Imaging the Bead-Loaded Cells
  • 04:43Results: Proteins and Nucleic Acids Bead-loaded onto Adherent Human Cells
  • 06:49Conclusion

Summary

Automatic Translation

珠子加载将蛋白质、质粒和颗粒引入粘附的哺乳动物细胞。这种细胞加载技术价格低廉、速度快,不会对细胞健康产生重大影响。它最适合活细胞成像。

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