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珠子将蛋白质和核酸加载到附体人类细胞中
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Bead Loading Proteins and Nucleic Acids into Adherent Human Cells
Please note that all translations are automatically generated.
Click here for the English version.
珠子将蛋白质和核酸加载到附体人类细胞中
DOI:
10.3791/62559-v
•
07:28 min
•
June 01, 2021
•
Charlotte Ayn Cialek
,
Gabriel Galindo
,
Amanda Lynn Koch
,
Matthew Neeley Saxton
,
Timothy John Stasevich
2
1
Department of Biochemistry and Molecular Biology
,
Colorado State University
,
2
World Research Hub Initiative, Institute of Innovative Research
,
Tokyo Institute of Technology
Chapters
00:04
Introduction
01:00
Clean, Sterilize, and Dry Glass Beads
02:26
Assemble the Bead Loader Apparatus
03:18
Bead Loading Cells and Imaging the Bead-Loaded Cells
04:43
Results: Proteins and Nucleic Acids Bead-loaded onto Adherent Human Cells
06:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
珠子加载将蛋白质、质粒和颗粒引入粘附的哺乳动物细胞。这种细胞加载技术价格低廉、速度快,不会对细胞健康产生重大影响。它最适合活细胞成像。
Tags
Bead Loading
Adherent Cells
Single Cell Microscopy
Proteins
Nucleic Acids
Cell Loading
Sodium Hydroxide
Ethanol
Biosafety
Polypropylene Mesh
UV Sterilization
Desiccant
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