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Direkte påvisning af isolevuglandiner i væv under anvendelse af et D11 scFv-alkalisk fosfatasefusionsprotein og immunofluorescens
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Biology
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JoVE Journal Biology
Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence

Direkte påvisning af isolevuglandiner i væv under anvendelse af et D11 scFv-alkalisk fosfatasefusionsprotein og immunofluorescens

DOI:
10.3791/62603-v

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06:33 min

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July 05, 2021

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, , , , , , ,
1Vanderbilt Eye Institute,Vanderbilt University Medical Center, 2Department of Cell and Developmental Biology,Vanderbilt University, 3Department of Biochemistry,Vanderbilt University, 4Division of Clinical Pharmacology, Department of Medicine,Vanderbilt University Medical Center, 5Division of Hematology and Oncology,Indiana University School of Medicine, 6Department of Molecular Physiology and Biophysics,Vanderbilt University

Chapters

  • 00:05Introduction
  • 00:54Immunofluorescence
  • 03:21Negative Controls and Competitive Assays
  • 04:37Results: Detection of IsoLGs in Hypertensive and Normotensive Mice and Human Tissues
  • 06:08Conclusion

Summary

Automatic Translation

Automatic Translation

Denne artikel indeholder en detaljeret metode til måling af isolevuglandiner i væv ved immunofluorescens under anvendelse af alkalisk phosphatase-konjugeret ScFv D11-antistof. Hypertensionsmodeller hos både mus og mennesker bruges til at forklare de trinvise procedurer og grundlæggende principper forbundet med isolevuglandinmåling i vævsprøver.

Tags

IsolevuglandinsD11 ScFv-alkaline PhosphataseImmunofluorescenceCardiovascular DiseaseAntigen RetrievalAlkaline Phosphatase DevelopmentImmunohistochemistryFluorescent Microscopy

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