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The Use of the Patch-Clamp Technique to Study the Thermogenic Capacity of Mitochondria
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JoVE Journal Biology
The Use of the Patch-Clamp Technique to Study the Thermogenic Capacity of Mitochondria

The Use of the Patch-Clamp Technique to Study the Thermogenic Capacity of Mitochondria

DOI:
10.3791/62618-v

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11:05 min

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May 03, 2021

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1Department of Physiology, David Geffen School of Medicine,University of California Los Angeles

Chapters

  • 00:04Introduction
  • 01:06Mitochondrial Isolation from the Mouse Heart
  • 03:01Preparation of Mitoplasts with a French Press for Mechanical Rupture of the OMM
  • 04:07Electrophysiological Recordings of H+ Leak Through UCP1 and AAC
  • 08:20Results: The Analysis of FA-Dependent Proton Current via UCP1 in Brown Fat and via AAC in Heart Mitoplast
  • 10:05Conclusion

Summary

Automatic Translation

Automatic Translation

This method article details the main steps in measuring H+ leak across the inner mitochondrial membrane with the patch-clamp technique, a new approach to study the thermogenic capacity of mitochondria.

Tags

Keywords: Patch-clampMitochondriaThermogenic CapacityProton LeakInner Mitochondrial MembraneCalciumAdenine NucleotidesMouseHeartHomogenizationCentrifugationFrench Press

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