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Tæthedsgradient ultracentrifugering til undersøgelse af endoytisk genanvendelse i pattedyrceller
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Biochemistry
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JoVE Journal Biochemistry
Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells

Tæthedsgradient ultracentrifugering til undersøgelse af endoytisk genanvendelse i pattedyrceller

DOI:
10.3791/62621-v

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05:13 min

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June 30, 2021

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, ,
1School of Life Sciences,The Chinese University of Hong Kong

Chapters

  • 00:05Introduction
  • 00:33Mammalian Cell Culture, Transfection and Cell Harvest
  • 02:07Density Gradient Column Preparation
  • 02:46Fractionation and Recovery of Recycling Endosome-Containing Fraction
  • 03:17Results: Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells
  • 04:47Conclusion

Summary

Automatic Translation

Automatic Translation

Dette papir har til formål at præsentere en protokol til forberedelse af genanvendelse endosomes fra pattedyr celler ved hjælp af saccharosetæthed gradient ultracentrifugering.

Tags

Density Gradient UltracentrifugationEndocytic RecyclingMammalian CellsProtein TraffickingRecycling EndosomesCell FractionationSucrose GradientPost-nuclear SupernatantHomogenization BufferDounce HomogenizerProtease InhibitorPhosphatase Inhibitor

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