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Enkeltcellekarakterisering af calciumtilstrømning og HIV-1-infektion ved hjælp af en multiparameter optofluidisk platform
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1Department of Medicine, Division of Infectious Diseases,Icahn School of Medicine at Mount Sinai, 2Department of Genetics and Genomic Sciences,Icahn School of Medicine at Mount Sinai, Icahn Institute of Data Science and Genomic Technology, 3Black Family Stem Cell Institute, Department of Genetics and Genomic Sciences,Icahn School of Medicine at Mount Sinai, Icahn Institute of Data Science and Genomic Technology, 4Sema4, a Mount Sinai venture
Chapters
- 00:05Introduction
- 00:57Preparation of Cells for Imaging
- 02:33Optofluidic System Preparation, Cell Loading, and Cell Penning
- 04:27Single-cell Infection and Functional Imaging
- 05:24Results: Single-Cell Characterization of Calcium Influx and HIV-1 Infection
- 06:20Conclusion
Her præsenterer vi en protokol, hvor enkelte celler overvåges for akutte hændelser og produktiv HIV-1-infektion på en nanofluidisk enhed. Billeddata definerer virusværtsreceptorinteraktioner og signalvejsdynamik. Dette er den første metode til nanofluidic high-throughput langsgående enkeltcellekultur og billeddannelse til at studere signalering af kinetik og molekylære interaktioner.
