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Developmental Biology
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基于2D和3D人诱导多能干细胞的模型,用于剖析新皮质发育过程中原发性纤毛受累
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Developmental Biology
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JoVE Journal
Developmental Biology
2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development
Please note that all translations are automatically generated.
Click here for the English version.
基于2D和3D人诱导多能干细胞的模型,用于剖析新皮质发育过程中原发性纤毛受累
DOI:
10.3791/62667-v
•
14:19 min
•
March 25, 2022
•
Lucile Boutaud*
1
,
Marie Michael*
1
,
Céline Banal
,
Damelys Calderon
,
Sarah Farcy
,
Julie Pernelle
,
Nicolas Goudin
,
Camille Maillard
,
Clémantine Dimartino
,
Cécile Deleschaux
,
Sébastien Dupichaud
,
Corinne Lebreton
,
Sophie Saunier
,
Tania Attié-Bitach
5
,
Nadia Bahi-Buisson
6
,
Nathalie Lefort
,
Sophie Thomas
1
Imagine Institute, INSERM UMR 1163
,
Université de Paris
,
2
Imagine Institute, iPSC Core Facility, INSERM UMR U1163
,
Université de Paris
,
3
Necker Bio-image Analysis platform of the SFR Necker
,
4
Imagine Institute, Cell Imaging Platform, INSERM-US24-CNRS UMS 3633 Structure Fédérative de Recherche Necker, INSERM UMR U1163
,
Université de Paris
,
5
Fédération de Génétique, Hôpital Necker-Enfants Malades
,
Assistance Publique Hôpitaux de Paris
,
6
Pediatric Neurology
,
APHP- Necker Enfants Malades Hospital
Chapters
00:07
Introduction
01:42
Generation of 2D hIPS Cell-Based Models of Neocortical Development
02:56
Generation of Dorsal Forebrain Organoids
04:56
In toto
Immunolabeling, Clearing, and Lightsheet Acquisition of Dorsal Forebrain Organoids
07:00
Immunostaining and Clearing of Free-Floating Sections of Dorsal Forebrain Organoids
09:20
Representative Results
13:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了基于2D和3D人类诱导多能干细胞(hIPSC)的新皮质发育模型的生成和表征的详细方案,以及能够对原发性纤毛(PC)生物发生和功能进行定性和定量分析的补充方法。
Tags
2D
3D
Human
Induced Pluripotent Stem Cell
Primary Cilium
Neocortical Development
Neural Rosettes
Dorsal Forebrain Organoids
Embryoid Body
Dual SMAD Inhibition
Immunohistochemistry
Vibratome
Confocal Microscopy
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