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In vivo Vurdering af Microtubule Dynamics og Orientering i Caenorhabditis elegans Neuroner
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Neuroscience
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JoVE Journal Neuroscience
In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons

In vivo Vurdering af Microtubule Dynamics og Orientering i Caenorhabditis elegans Neuroner

DOI:
10.3791/62744-v

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07:43 min

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November 20, 2021

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,
1DBT-National Brain Research Centre

Summary

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En protokol til billeddannelse af de dynamiske mikrotubuler in vivo ved hjælp af fluorescerende mærket End bindende protein er blevet præsenteret. Vi beskrev metoderne til at mærke, billede, og analysere de dynamiske mikrotubuler i Posterior lateral microtubule (PLM) neuron af C. elegans.

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Keywords: Microtubule DynamicsMicrotubule OrientationCaenorhabditis Elegans NeuronsIn Vivo AssessmentCytoskeletonAlpha And Beta TubulinEnd-binding ProteinsEBP-2GFPPLM NeuronsMake-3 PromoterMountingPolystyrene BeadsFluorescence MicroscopySpinning Disk Unit

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