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Biochemistry
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用于单颗粒低温电子显微镜的生物标本的手动印迹和切入式冷冻
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Biochemistry
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Biochemistry
Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
用于单颗粒低温电子显微镜的生物标本的手动印迹和切入式冷冻
DOI:
10.3791/62765-v
•
09:16 min
•
February 07, 2022
•
Hoang P. M. Nguyen*
1
,
Kelly L. McGuire*
1
,
Brian D. Cook*
1
,
Mark A. Herzik, Jr.
1
Department of Chemistry and Biochemistry
,
University of California, San Diego
Chapters
00:04
Introduction
00:22
Prepare the Manual Plunging Environment
00:42
Prepare the Cryogen Dewar and Manual Plunger
02:04
Prepare the Cryogen
04:36
Prepare Plunging Materials and Accessories
05:13
Prepare CryoEM Specimens by Plunge Freezing
07:22
Representative Results
07:50
Conclusion
Summary
Automatic Translation
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Automatic Translation
本手稿概述了手动冷冻生物标本以进行单颗粒低温电子显微镜的印迹和暴跌方法。
Tags
Keywords: Manual Blot-and-plunge Freezing
Biological Specimens
Single-particle Cryogenic Electron Microscopy
Transmission Electron Microscopy
Cryogen Dewar
Manual Plunger
Ethane Vessel
Plunging Arm
Cryogen Preparation
Liquid Nitrogen
Ethane Gas
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