Journal
/
Biology
/
Isolering af menneskelige neutrofiler fra fuldblod og buffy frakker
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Isolation of Human Neutrophils from Whole Blood and Buffy Coats

Isolering af menneskelige neutrofiler fra fuldblod og buffy frakker

DOI:
10.3791/62837-v

•

08:12 min

•

September 17, 2021

•

, , ,
1Department of Pathology,Harvard Medical School, Department of Lab Medicine, Children’s Hospital Boston, and Dana-Farber/Harvard Cancer Center, 2Department of Microbiology, Faculty of Science,Mahidol University

Chapters

  • 00:04Introduction
  • 00:45Gradient Layering
  • 02:40Erythrocyte Sedimentation
  • 03:45Residual RBC Lysis
  • 05:06Quality Determination of Isolated Neutrophil
  • 05:44Results: Neutrophil Yield and Quality Estimation
  • 07:34Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver en metode til isolering af neutrofiler fra fuldblod, buffy frakker, eller leukaferse membraner, opnå godt udbytte, høj renhed, og minimal celle aktivering. Vi bruger gradient rensning, rød blodlegemer (RBC) sedimentering, og RBC lysis at opnå en høj kvalitet / renhed neutrophil forberedelse.

Tags

NeutrophilsDensity GradientSedimentationLysisBuffy CoatPBMCErythrocyteCell BiologyCell IsolationCell Preparation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62837?article_type=v

Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies
check_url/62837?article_type=v

Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice
check_url/62837?article_type=v

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin
check_url/62837?article_type=v

Naive voksne stamceller Isolering fra primære humane fibroblastkulturer
check_url/62837?article_type=v

Manuel Isolering af Adipose-afledte stamceller fra humane Lipoaspirates
check_url/62837?article_type=v

Isolering af Primære myofibroblasts fra mus og Human tyktarmsvæv
check_url/62837?article_type=v

Isolering af blod-fartøj-afledt Multipotente Forstadier fra human skeletmuskel
check_url/62837?article_type=v

Isolering af Small noncoding RNA'er fra human serum
check_url/62837?article_type=v

Isolering af myofibroblaster fra mus og human Spiserøret
check_url/62837?article_type=v

Generation og Kultur of Blood Udvækst endotelceller fra human perifert blod

Read Article