Journal
/
Biology
/
Fremstilling af multifunktionelle silkebaserede mikrokapsler fyldt med DNA-plasmider, der koder for RNA-aptamerere og ribotekser
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Fremstilling af multifunktionelle silkebaserede mikrokapsler fyldt med DNA-plasmider, der koder for RNA-aptamerere og ribotekser

DOI:
10.3791/62854-v

•

10:07 min

•

October 08, 2021

•

, , ,
1UES Inc., 2711th Human Performance Wing, Airman Systems Directorate, Air Force Research Laboratory,Wright-Patterson AFB, 3Materials and Manufacturing Directorate,Wright-Patterson AFB

Chapters

  • 00:04Introduction
  • 00:56Perform Layer‐by‐Layer Deposition of a Prime Layer, DNA Plasmids, and Silk Layers
  • 04:44Dissolution of SiO2Cores and Imaging of Silk Fibroin Microcapsules
  • 05:42Estimation of the Permeability of Hollow Microcapsules Using Molecular Weight Cut‐Off (MWCO) Method
  • 06:42In Vitro Activation of Synthetic Theophylline Riboswitch in Silk Microcapsules
  • 07:28Results: Microscopy Studies, Membrane Permeability, and Gene Expression Kinetics of Silk Fibroin Microcapsules
  • 09:25Conclusion

Summary

Automatic Translation

Automatic Translation

Protokollen beskriver dannelsen af robuste og biokompatible DNA-belastede mikrokapsler som multipleksede in vitro-biosensorer, der er i stand til at spore flere ligander.

Tags

Keywords: Multifunctional Silk-based MicrocapsulesDNA PlasmidsRNA AptamersRiboswitchesBiocompatible MicrocapsulesDNA ImmobilizationBiosensorsBiological ProcessesSignaling MoleculesGene ActivationPolyethyleneimineSilica MicroparticlesDNA PlasmidsTheophylline RiboswitchBroccoli AptamerSilk Fibroin

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62854?article_type=v

Homemade Site Directed Mutagenesis of Whole Plasmids
check_url/62854?article_type=v

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
check_url/62854?article_type=v

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
check_url/62854?article_type=v

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
check_url/62854?article_type=v

In vitro Transskription og lukning af Gaussia luciferase-mRNA Efterfulgt af HeLa Celletransfektion
check_url/62854?article_type=v

Fremstilling af DNA-tværbundet polyacrylamid Hydrogeler
check_url/62854?article_type=v

Real-time Imaging af Single Engineered RNA-transkripter i levende celler Brug Ratiometrisk bimolekylær Beacons
check_url/62854?article_type=v

Reduceret tyngdekraft Miljø Hardware Demonstrationer af en prototype miniaturiseret flowcytometer og Companion Mikrofluid Blanding Technology
check_url/62854?article_type=v

Forstærkning, Næste generations sekventering, og Genomisk DNA Kortlægning af retroviral integration steder
check_url/62854?article_type=v

Visualisering af Surface-tøjret store DNA-molekyler med et fluorescerende protein DNA-bindende peptid

Read Article